The state of the Kenzan methodology of scaffold-free 3D bioprinting in 2020

A latest progress report printed within the journal Superior Healthcare Supplies particulars the outcomes of assorted experiments which have utilized the Kenzan methodology of 3D bioprinting to provide cell constructs for transplantation. The paper is meant to provide readers an summary of the state-of-the-art know-how in 2020.

Because the flip of the millennium, analysis into 3D bioprinting has gained some severe traction. One of many extra developed strategies of bioprinting, the Kenzan methodology, includes skewering a tradition of cells referred to as spheroids on an array of microneedles (the Kenzan) and ready for them to partially fuse. As soon as the cells are capable of help their very own construction the needles are retracted and the cells are nurtured and grown right into a usable tissue patch.

In keeping with the pioneers of the tactic at Saga College, it’s the solely methodology of 3D bioprinting surgically transplantable cell-only buildings with out the necessity for synthetic components. The cell-only nature of the Kenzan methodology qualifies it as a ‘scaffold-free’ course of as the one supporting construction of the bioink is the array of microneedles.

A tubular cell structure being 3D printed with the Kenzan method. Image via Saga University.A tubular cell construction being 3D printed with the Kenzan methodology. Picture through Saga College.

Peripheral nerve reconstruction

If the nerve hole lengthens considerably after a peripheral nerve damage, an autologous nerve transplant could also be required. This primary-line-of-defense remedy does have its downsides, nevertheless. The therapeutic over of the transplant could lead to inconsistencies and neuroma formation which is problematic and requires additional remedy. Subsequently, Yurie et al. tried to reconstruct scaffold-free mobile nerve conduits for the peripheral nerve of a rat.

Utilizing dermal fibroblasts – the spheroids on this case – taken solely from a human, the crew 3D bioprinted the tubular cell constructs and implanted them on the sciatic nerve of a rat. The nerves had been nonetheless linked eight weeks after the process and useful neurons might be noticed within the nerve hole. The researchers concluded that the transplanted tubular buildings had promoted useful nerve reconstruction on the web site of the sciatic nerve.

Nerve reconstruction. Image via Saga University.Nerve reconstruction. Picture through Saga College.

Purposeful coronary heart tissue reconstruction

Right this moment’s remedies for coronary heart failure are inclined to solely relieve signs for a comparatively quick time and delay the inevitable, because the reconstruction or therapeutic of useful coronary heart tissue will not be but established. Ong et al. tried to make use of the Kenzan methodology to reconstruct useful, wholesome cardiac tissue to fix a (damaged) rat coronary heart.

The spheroids on this experiment included cardiomyocytes derived from pluripotent stem cells and ventricular cardiac fibroblasts – each sourced from people. As soon as transplanted, the researchers noticed a spontaneous pulsation of the cell assemble. Full engraftment was confirmed every week after the surgical procedure and the transplanted coronary heart tissue was discovered to contribute on to the performance of a rat coronary heart.

Heart reconstruction. Image via Saga University.Coronary heart reconstruction. Picture through Saga College.

Esophagus reconstruction

Present strategies of esophageal reconstruction post-esophagectomy more-often-than-not lead to issues and have a excessive mortality charge. To deal with this, Takeoka et al. tried to 3D bioprint esophagus-like constructs to be transplanted into rats and noticed the outcomes.

The spheroids used on this experiment included human dermal fibroblasts and human esophageal easy muscle cells. As soon as the tubular tissue buildings had been fabricated, they had been implanted and absolutely engrafted after 30 days. The researchers discovered that the rats may efficiently go meals by way of their new foodpipes into their stomachs with none issues. The crew concluded that the experiment “exhibited promise” and expects to scale this analysis as much as a human process in the future.

Esophagus reconstruction. Image via Saga University.Esophagus reconstruction. Picture through Saga College.

An in depth clarification of the Kenzan methodology and its in depth purposes will be discovered within the paper titled ‘Scaffold‐Free Bio‐3D Printing Utilizing Spheroids as “Bio‐Inks” for Tissue (Re‐)Development and Drug Response Exams’. It’s co-authored by Daiki Murata, Kenichi Arai, and Koichi Nakayama.

Whereas the Kenzan methodology is exclusive in its scaffold-free nature, different strategies of 3D bioprinting have additionally seen success lately. In Korea, CLECELL, a 3D bioprinting startup, has developed a respiratory epithelium mannequin utilizing its proprietary 3D bioprinting know-how. The crew intends to make use of the mannequin as a testbed for numerous viruses to higher perceive their conduct. Elsewhere, on the ISS, nScrypt has accomplished the primary useful 3D bioprinting experiment within the microgravity of house – a human knee meniscus.

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Featured picture reveals the Kenzan methodology and its many purposes. Picture through Saga College.

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