A workforce of scientists from UC Berkeley, California, have proposed a brand new 3D printing methodology for producing synthetic tissues. Described to The Every day Californian as “like making a hamburger in a really cool — cryogenic — answer,” they imagine this various course of could possibly be key to growing the pace of 3D fabrication.
The outcomes from a current research of the tactic by the workforce have been just lately printed in the ASME’s Journal of Medical Units. It describes the fundamental ideas of the so-called multilayer cryolithography (MLCL) know-how, and its viability for producing easy, coherent buildings.
How is 3D bioprinting like making a hamburger?
Although likened in some ways to 3D bioprinting processes, UC Berkeley’s MLCL approach is definitely a form of deconstructed 3D printing course of. As an alternative of increase sequential layers of fabric till a construction is made, MLCL truly breaks up this course of right into a collection of particular person 2D layers. The 2D layers of fabric are deposited parallel to 1 one other and concurrently frozen. They’re then assembled into the 3D construction and sure collectively.
Step-by-step of the UC Berkeley multilayer cryolithography methodology. Picture by way of ASME Journal of Medical Units
A clearly finicky course of Gideon Ukpai, one of many research’s co-authors explains, “We had some challenges with making an attempt to move the layers and assembling them. The layers binding collectively after the actual fact was additionally a problem.”
Zichen Xiao nonetheless, one other of the venture’s researchers who likened the method to a frozen hamburger, described the success as, “You already print a number of layers, and also you mainly choose up one layer and stack them on prime of one another. You place the underside bread there first and put no matter layer you need on prime of it.”
One of many essential benefits of this course of over extra standard 3D bioprinting processes that use bioinks and hydrogels, is that the matter is ready to retain its form. “By the method of freezing,” Xiao provides, “it retains its inflexible construction, and the cells are nonetheless alive.”
Within the experiment, a construction containing 10 layers was made utilizing MCLC on a modified Flashforge 3D printer. The outcomes exist as a proof of idea for the tactic, and supply the workforce with a basis for future work.
Advanced ring and grid shapes fabricated utilizing UC Berkeley’s multilayer cryolithography methodology. Picture by way of ASME Journal of Medical Units
Cryogenic 3D bioprinting
So – is freezing the reply to higher, sooner 3D bioprinting? Different researchers are exploring using cryogenics in 3D bioprinting, together with a workforce from Imperial School London within the UK. Nonetheless the improved efficiency promised by such strategies remains to be up for debate.
The subsequent step for the UC Berkeley workforce is to optimize the MCLC course of, characterize the merchandise, and in addition “decide the suitable situations that current essentially the most benefits.”
Full outcomes of this workforce’s most up-to-date research of the tactic, titled “A parallel a number of layer cryolithography gadget for the manufacture of organic materials for tissue engineering,” are printed on-line in ASME’s Journal of Medical Units. The paper is co-authored by Gideon Ukpai, Joseph Sahyoun, Robert Stuart, Sky Wang, Zichen Xiao and Boris Rubinsky.
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Featured picture reveals advanced ring and grid shapes fabricated utilizing UC Berkeley’s multilayer cryolithography methodology. Picture by way of ASME Journal of Medical Units
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